Bacteriophage Research Papers

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The findings more than double the number of known defense mechanisms, piquing the interests of molecular biology tool developers.

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Image of the Day: Virus Spacecraft 

By The Scientist Staff | January 5, 2018

Researchers reconstruct images of a virus that infects Escherichia coli bacteria.

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Bacteriophages to the Rescue

By Emily Monosson | July 17, 2017

Phage therapy is but one example of using biological entities to reduce our reliance on antibiotics and other failing chemical solutions.

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Book Excerpt from Natural Defense

By Emily Monosson | July 17, 2017

In Chapter 3, “The Enemy of Our Enemy Is Our Friend: Infecting the Infection,” author Emily Monosson makes the case for bacteriophage therapy in the treatment of infectious disease.

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AcrIIA4, an inhibitor protein from the Listeria bacteriophage, can block DNA from binding to Cas9 during genome editing.

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When engineered to use a four-billion-year-old version of the protein thioredoxin, the bacteria can stall bacteriophage replication, a new study shows.

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Viruses within Salmonella rapidly spread genes throughout the bacterial population during a gut infection, scientists show.

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A team finds that viruses can sense chemical signals and use them to decide whether to kill or infect their hosts.

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Keeping CRISPR in Check

By Anna Azvolinsky | December 14, 2016

In bacteriophage genomes, researchers find three anti-CRISPR proteins that naturally inhibit CRISPR-Cas9 in one bacterial species and can do the same in human cells. 

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Researchers find evidence of antibiotic resistance genes in the DNA of viruses that infect bacteria.

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Bacteriophages, viruses that infect bacteria, are the planet's most numerous microorganisms. They kill vast numbers of bacteria in natural environments. They can also be used to treat bacterial infections.

In the 1920s and 1930s, before antibiotics became widely available, physicians in the United States and other countries successfully treated a variety of infections with bacteriophages (“phage therapy”). Phage therapy continues to be used in certain countries, including Georgia and Poland, but in the United States and most of Europe, phages were set aside with the rise of antibiotics, which were seen as a magic bullet. Now, with alarm bells ringing louder than ever over the rise in antibiotic resistance, scientists are turning to different avenues to attack bacterial infections. One of these is phage therapy.

Unlike many antibiotics, bacteriophages only infect specific bacteria and are typically harmless to the host organism, including the beneficial bacteria of the host. Antibiotic-resistant bacteria are usually fully sensitive to phages, and because phages are so abundant in nature, multiple phages can be used together or combined with antibiotics to maximize treatment outcomes.

However, new research conducted at the University of Miami in Coral Gables, Florida and the National Cancer Institute, in Bethesda, Maryland demonstrate that a subset of bacteriophages, dubbed “superspreaders,” potentially play a major role in transmitting antimicrobial resistance. The research, described in mBio, provides an answer to a long-standing biological question and may help scientists better select specific bacteriophages for therapeutic use.

mBio Report: Novel "Superspreader" Bacteriophages Promote Horizontal Gene Transfer by Transformation

"Our work suggests that phage superspreaders may help drive bacterial evolution in natural environments but are not suitable for use in medicine," said lead study author Eric Keen, a graduate student at Washington University in St. Louis, Missouri, who conducted the project at the University of Miami in Coral Gables, and at the NCI, in Bethesda, Maryland. “We think our findings are something that clinicians and researchers should be aware of because different phages seem to disseminate antibiotic resistance genes at different rates. Obviously, we don’t want to be designing therapies using phages which are efficient at spreading antibiotic resistance genes across a bacterial community.”

While scientists have known that many bacteria carry plasmids, extrachromosomal DNA elements that frequently encode antibiotic resistance, nobody had previously studied what happens to these plasmids when an invading bacteriophage blows apart a bacterial cell. "It has not been known whether plasmids are destroyed during phage infection or released intact upon phage lysis, whereupon their encoded resistance could be acquired by other bacteria," said Mr. Keen.

To solve this knowledge gap, Mr. Keen and colleagues assembled a library of environmental phage isolates from environmental samples, including soil samples from Maryland and canal water in Florida. They infected Escherichia coli strains resistant to the antibiotic ampicillin with 20 different phage isolates and then measured the amount of plasmid DNA released intact upon phage lysis.

The researchers found that two of the 20 bacteriophages promoted plasmid transfer 50-fold in excess of that seen with the other 18 phages and that these two phages were able to efficiently release several different plasmids, each carrying a different antibiotic resistance gene. The researchers called these two bacteriophages SUSP1 and SUSP2, short for superspreader. “We coined that name because we thought what we were seeing was evocative of a phenomenon in epidemiology in which you have an outsized transmission by a relatively small percentage of the overall population,” said Mr. Keen.

Additional experiments demonstrated that SUSP2 encouraged the transfer of antibiotic resistance to communities of soil bacteria from Maryland and Wyoming. Adding SUSP2 to co-cultures of kanamycin-resistant E. coli and kanamycin-sensitive Bacillus bacteria resulted in roughly 1,000-fold more kanamycin-resistant Bacillus bacteria than arose in phage-free controls, suggesting that this phage might be able transfer antibiotic resistance genes to bacteria besides E. coli and under more natural conditions.

The researchers characterized the biology of the two phages using electron microscopy and genome sequencing. "Based on our work with a number of different mutants of well-characterized phages, we hypothesize that the reason these two phages are special is because they lack hydrolytic endonucleases that other phages use to chop up DNA very efficiently during infection," said Mr. Keen. "We think that superspreaders lack these enzymes, and as a result DNA survives their infection basically intact."

He said researchers should steer clear of the “superspreaders” when designing bacteriophage therapies, noting that the majority of phages tested were far less efficient at promoting antibiotic resistance transfer. Future research directions include a more comprehensive assessment of the specific underlying mechanisms of the superspreaders and an evaluation of the prevalence of the phenomenon in natural environments, including in the human body.

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